Our next step in preparation of our slide requires us to navigate through the image. If you click and drag with your mouse, you can pan the image. The scroll wheel on your mouse or pinch zoom with the track pad on a laptop can be used like on any touch screen. (There is also a zoom bar on the left side that you can use to zoom in if pinching the track pad is not working).
There are also 3 preset zoom settings by the zoom bar, which are helpful when navigating to a certain zoom level.
There will be green lines around the areas the Grundium automatically detects as samples, so if it is very close, just click and drag those lines to expand the area over the entire sample.
It is advised that not too much time is spent trim very small areas, because eventually the time saved scanning is less then the time it takes to trim them in the first place
If the automatically generated lines do not fully cover the sample, select the border, once selected it will turn BLUE then click on “remove” tool to delete the lines, and then use the “add” tool to click and drag a box around the sample(s).
Then click the “Trim all” tool to trim the white space around each sample (too much white space makes each scan considerably longer) and make sure that no parts of the sample are cut off. If they appear to be cut off, click and drag the edge of the green lines to cover the sample.
Clicking on the “Remove” button deletes all green select lines.
If specific lines need to be deleted, click on the green line you want to delete, and it will turn blue. This means it is selected. (Clicking anywhere off of the screen deselects) Now click “Remove” and it will delete those lines you have selected.
If you are having trouble selecting lines, try refreshing the page
Now we need to make sure the focus is correct on our sample. Make sure the piece of sample is in the center of the screen, then zoom into 20x using the zoom presets in the top left corner.
Click the button “start” next to “live view” This will bring up a live view of the sample.
To focus properly on our sample, Turn the knobs on the side of the microscope and adjust our coarse focus.
Move to sample using “Navigator” view to monitor coarse focus, When the microscope says, “focus in range”, click the “AF” (auto focus) button in the bottom right corner of the live view window.
Once we have the sample in focus, we are now ready to scan.
NOTE: Make sure you are in range of the actual SAMPLE, and not the cover slide or glass backing slide. You need to be very careful when focusing, the Grundium may say “focus in range” when it thinks it is in focus.