- If this is your first time setting up the software, you will need to create presets. If the presets are accidently modified, you can go through these steps again to correct them. To start, click the “Adjust Scan” button on the top right of the screen.
- Beginning with the 4x objective engaged (red ring), enter the magnification of the objective (4.0) in the “Objective Lens Magnification” box. Next enter the magnification of the camera adapter (O.63). To save this preset, hit the “+” symbol and type “4x Magnification (Red Ring)” into the preset. Press the save button.
- Now we need to perform a color balance to ensure that our sample is properly adjusted. To do this, we need to make sure that the red, green and blue lines overlap, and their peaks are visible on the histogram.
- Perform the “white balance” by moving the slide to an empty (white) area of the slide that has no specimen or label. Press the “magic wand” icon and the calibration will be done automatically.
- We can see that after proper calibration, the slide appears brighter and all our lines on the histogram match, giving us the best quality of scan.
- Be SURE to press the “save” button again to save this white balance calibration to the preset.
- Now repeat this process with the 20x Magnification (Green Ring). You now have set up the presets. Whenever you physically change Magnifications (Objectives), you will simply select the correct magnification from the dropdown. Both the physical and dropdown selections are show in the following photos.